CHROMATOGRAPHIC AND PHOTOCOLORIMETRIC DETERMINATION OF TRICHLORMETAPHOS-3 IN ENVIRONMENT

Abstract

Purpose. To study conditions of chemical separation from vegetable raw materials (sugar-beet) and to accomplish the purification of the obtained extracts as well as to carry out qualitative and quantitative analyses of trichlormetaphos- 3 in the extracts. Methodology. We have used a mixture of chloroform and acetone for the separating of trichlormetaphos-3 and have performed the purification of pesticide by means of chloroform extraction. We have applied a thin layer chromatography and gas chromatographic method for the identification as well as a photocolorimetry (PhEC-56М, cuvette wide – 10 cm, red color filter) for quantitative determination of trichlormetaphos- 3. Results. We have extracted trichlormetaphos-3 from crushed sugar beet using a mixture of chloroform and acetone and have used the dry residue of extract for mineralization and for the consequent quantitative detection of trichlormetaphos-3 by a thin layer chromatography (mixture solvent is chloroform – benzene (1:1), the developer – 25% aqueous solution of ammonia and 2% aqueous solution of 4-aminoantypiryn). We have performed a gaschromatographic analysis of trichlormetaphos-3 on Chrome-5 chromatograph (duration of analysis has been 7 min) as well as have carried out the photocolorimetric analysis of pesticide by measuring the optical density of the prepared solution and using reactions of ammonium molibdate and benzidine. Originality. For the first time, we have elaborated the methods of separation, purification, qualitative and quantitative determination of trichlormetaphos-3 in food raw materials. Practical value. We have proposed the technique of fast identification and determination of extremely toxic pesticide relevant to the group of phosphorus compounds – trichlormetaphos-3 in liquids, air and food raw materials. References 9, scheme 1, figures 1.