COMPLEX COMBINATION OF ANALYTICAL METHODS FOR THE QUANTITATIVE DETERMINATION OF FLUACIZINE IN SOLUTIONS, BIOLOGICAL MATERIAL AND AIR

Abstract

Identification techniques of fluacizine by color reactions and thin layer chromatography were elaborated. The results of chromatographic analysis of fluacizine for two systems of solvents (methanol – ammonia and benzene – methanol – acetic acid) reveal that necessary interval for the chromatogram developing is 35 min, the detection limit is 1 mg, disappearance of spots on plates occurs in 30-40 min and the Rf value depends on purity and qualification of dissolvents. The fluacizine detection and determination has been fulfilled by UV-spectrophotometry in phosphate buffer solution at 256 nm. The kept up to date analysis methods of the spectrophotometric and gel-chromatographic analysis were used for techniques elaboration of the quantitative determination of fluacizine in solutions, biological material and air.