TECHNIQUE IMPROVEMENT OF ETHANOL CHROMATOGRAPHING AND ITS DETECTION IN BIOLOGICAL FLUIDS AND AIR

Abstract

Purpose. To have elaborated the accurate and reproducible techniques of extraction, purification and qualitative assay for ethanol qualitative analysis in biological fluids (urine) and ambient air (out of working area) by means of gasliquid chromatography (GLC) and liquid chromatography (LC) methods. Methodology. For choice of the optimum gascarrier velocity, we have prepared a test reagent mixture. To this end, we have used alkyl nitrite technique of ethanol detection. We have applied a gas-liquid chromatography and liquid chromatography method for qualitative determination of ethanol in biological fluids and air. Results. We have taken urine from drunken person then have adjusted specimen to pH 9.0 by addition of 20% solution of Na2CO3 and have extracted ethanol by chloroform two times in 10 mL. Besides, we have filled an aspirator (or rubber bulb) with fifty liters of contaminated air with ethanol taken from working area. We have carried out the gas-liquid chromatography and the liquid reversed-phase chromatography for the extracts analyses out of urine and research of contaminated air. But formerly, we have calculated the graphic curves for selecting the optimal gas-carrier velocity using the dependencies based on the theory of gas-carrier velocity in GLC method. We have experimentally ascertained that the duration of LC analysis (1 min and 36 s) of urine (or of air) polluted with ethanol is at least two time less than it was for LGC analysis (4 min and 15 s). Originality. For the first time, we have elaborated the accurate and reproducible techniques of extraction, purification and qualitative assay for ethanol qualitative analysis in biological fluids (urine) and ambient air (out of working area). Practical value. The obtained results are well-reproducible, both introduced techniques are accurate, sensitive and demonstrative; the methods of LGC and LC analyses can be successfully used to detect ethanol in biological fluids and ambient air. References 15, tables 1, figures 2.